VIDEO ANSWER: Hello. In this question it has been asked, competent cells are rendered permissible by exposing them to calcium chloride solution. Why do you think the competent cells must be kept on ice during transformation? Okay that's the question.
Competent cells are rendered permeable by a solution: calcium chloride transformation. Competent cells must be kept on ice during cooling.
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SOLVED: When preparing competent cells for transformation, you first washed them in cold MgCl2 and then in cold CaCl2. What is the purpose of these salt solutions? If you forget to heat
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SOLVED: Competent cells are rendered permeable by a solution: calcium chloride transformation. Competent cells must be kept on ice during cooling.
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