Fans theorize the possibility of Gwen Stacy from Spider-Man: Across the Spider-Verse being Transgender due to potential sub-text.
Gwen (2018) - News - IMDb
Are they saving them for the 3rd one?: Spider-Man: Across the Spider-Verse Boss Signals
I Agree That 'Spider Girl' AKA Gwen Stacy Is Trans In 'Across The Spiderverse
Gwen is blatantly a trans woman in this movie: Across the Spider-Verse Spider-Gwen Fan Theory Changes Everything
Dina Reviews: Across the Spider-Verse - The Art of Dina M
Gwen is blatantly a trans woman in this movie: Across the Spider-Verse Spider-Gwen Fan Theory Changes Everything
Spider-Man: Across the Spider-Verse (Western Animation) - TV Tropes
Spider-Man: Across the Spider-Verse' fans see Gwen Stacy as trans. Why it matters
Gwen is blatantly a trans woman in this movie: Across the Spider-Verse Spider-Gwen Fan Theory Changes Everything
Is Across the Spider-Verse's Gwen Stacy Trans?
Spider-Verse' Sequel Banned In Saudi Arabia, UAE Over Trans Flag: Report
Gwen's isn't the only trans narrative
Spider-Man: Across the Spider-Verse, OT, Party Like It's 2099 [Open Spoilers], Page 52